Cruz Hewitt, the 16-year-old son of Australian legend Lleyton, also lost on the second day of qualifying.
Watched by his father on a packed court of three, Hewitt lost 6-1, 6-4 to former world No. 16 Nikoloz Basilashvili of Georgia.
Australia’s Bernard Tomic, previously a top-20 player, also fell short, beaten 6-3, 6-1 by Slovakian 21st seed Jozef Kovalik.
Britain’s Harriet Dart and Heather Watson made it through to the second round of qualifying on Monday.
Katie Boulter, Emma Raducanu, Sonay Kartal, Jodie Burrage, Jack Draper, Cameron Norrie and Jacob Fearnley all earned direct entry into the main draw.